Products: Fluid Solutions

• Without calcium and magnesium (Cat # 2009-5)
• With calcium and magnesium (Cat # 2009-5CM)
• Provided in 500ml plastic bottles

2 year shelf life

UPPER LAYER (45%)

• Cat # 2221-100 100ml
• Cat # 2221-50 50ml

LOWER LAYER (90%)

• Cat # 2222-100 100ml
• Cat # 2222-50 50ml

Ready-to-use density gradient system designed to separate the motile fraction of human sperm from seminal fluid

COMPONENT LIST
Sodium Chloride
Potassium Chloride
Magnesium Sulfate, Heptahydrate
Potassium Phosphate, Monobasic, Anhydrous
Calcium Chloride, Dihydrate
Sodium Bicarbonate
Dextrose, Anhydrous
Sodium Pyruvate
Sodium Lactate
Alanyl Glutamine
Gentamicin
Phenol Red
HEPES, Sodium Salt
Silane Coated Colloidal Silica
Human Serum Albumin

• Silane-coated colloidal silica particles suspended in HEPES-buffered HTF
• Uses a combined Sodium Bicarbonate/HEPES buffering system which is appropriate for those procedures that do not use a carbon dioxide atmosphere
• Formulated with a stabilized form of glutamine
• Contains the antibiotic Gentamicin
• For use without CO2 (pre-equilibrate at 37ºC)
• Contains 5 mg/ml HSA
• Provided in both 50ml and 100ml glass bottles
• One-Cell Mouse Embryo tested (>80% 1-cell to expanded blastocyst within 96 hrs)

Store at 2-8ºC
2 year shelf life

For use as a fertilization medium and for early embryo culture through 2PN stage

COMPONENT LIST
Sodium Chloride
Potassium Chloride
Magnesium Sulfate, Heptahydrate
Potassium Phosphate, Monobasic, Anhydrous
Calcium Chloride, Dihydrate
Sodium Bicarbonate
Glucose, D- (+)
Sodium Pyruvate
Sodium Lactate
Alanyl Glutamine
EDTA, Tetra-Sodium Salt
Gentamicin
Phenol Red

• Uses a Sodium Bicarbonate buffering system which is appropriate for those procedures requiring the use of a carbon dioxide atmosphere during incubation
• Formulated with EDTA and a stabilized form of glutamine
• Contains the antibiotic Gentamicin
• Requires protein supplementation
• For use after pre-equilibrate at 37ºC in 5-6% CO2
• Provided in 100ml plastic bottles
• One-Cell Mouse Embryo tested (>80% 1-cell to expanded blastocyst within 96 hrs)

Store at 2-8ºC
120 day shelf life

Protein supplement for culture media applications requiring additional supplementation

• 100 mg/ml total protein (weight/volume) in normal saline
• Formulated with therapeutic grade Human Serum Albumin
• Parent HSA used to formulate this product is derived from blood from CBER licensed Blood Banks
• All donors used to obtain the Human Albumin were individually tested and found to be non-reactive for HbsAg, HCV and antibodies to HIV-1 and HIV-2 by approved testing methods. Source material is also screened by questionnaire at the donor level for risk factors associated with Creutzfeldt-Jakob Disease (CJD).
• For use after pre-equilibration at 37ºC
• Provided in 12x5ml glass vials/box
• One-Cell Mouse Embryo tested (>80% 1-cell to expanded blastocyst within 96 hrs)

Store at 2-8ºC
18 month shelf life

For use as a cleavage medium, Day 3 embryo transfers and ICSI procedures

• Low Glucose and Phosphate Free
• Uses a Sodium Bicarbonate buffering system which is appropriate for those procedures requiring the use of a carbon dioxide atmosphere during incubation
• Formulated with EDTA and a stabilized form of glutamine
• Contains the antibiotic Gentamicin
• Requires protein supplementation
• For use after pre-equilibrate at 37ºC in 5-6% CO2
• Provided in 20ml glass bottles
• One-Cell Mouse Embryo tested (>80% 1-cell to expanded blastocyst within 96 hrs)

Store at 2-8ºC
120 day shelf life

For egg retrieval, follicle flushing, ICSI procedures and cryopreservation

COMPONENT LIST
Sodium Chloride
Potassium Chloride
Magnesium Sulfate, Heptahydrate
Potassium Phosphate, Monobasic, Anhydrous
Calcium Chloride, Dihydrate
Sodium Bicarbonate
Glucose, D- (+)
Sodium Pyruvate
Sodium Lactate
Alanyl Glutamine
EDTA, Tetra-Sodium Salt
Gentamicin
Phenol Red
HEPES, Sodium Salt

• Uses a combined Sodium Bicarbonate/HEPES buffering system which is appropriate for those procedures that do not use a carbon dioxide atmosphere
• Formulated with EDTA and a stabilized form of glutamine
• Contains the antibiotic Gentamicin
• For use without CO2 (pre-equilibrate at 37ºC)
• Requires protein supplementation
• Provided in both 100ml and 500ml plastic bottles
• One-Cell Mouse Embryo tested (>80% 1-cell to expanded blastocyst within 96 hrs)

Store at 2-8ºC
18 month shelf life

For extended embryo culture and transfer through expanded Blastocyst (Day 5)

• Supplemented with Amino Acids and Vitamins
• Uses a Sodium Bicarbonate buffering system which is appropriate for those procedures requiring the use of a carbon dioxide atmosphere during incubation
• Does not contain EDTA
• Formulated with a stabilized form of glutamine
• Contains the antibiotic Gentamicin
• Requires protein supplementation
• For use after pre-equilibrate at 37ºC in 5-6% CO2
• One-Cell Mouse Embryo tested (>80% 1-cell to expanded blastocyst within 96 hrs)

Store at 2-8ºC
120 day shelf life

For use as an overlay when culturing in reduced volumes of media to prevent evaporation and to insulate the media from changes to osmolarity and pH

• Pharmaceutical grade sterile, light mineral oil
• Double pre-washed, ready-to-use
• For use after pre-equilibration at 37ºC
• Provided in both 100ml and 500ml glass bottles
• One-Cell Mouse Embryo tested (>80% 1-cell to expanded blastocyst within 96 hrs))

Store at 2-8ºC
18 month shelf life

For use in ICSI procedures involving immobilization of sperm

• 10% Polyvinylpyrrolidone in HEPES buffered HTF
• Ready-to-use solution
• Provides more control of fluid in the ICSI pipette and prevents sperm from sticking to the injection pipette
• Uses a combined Sodium Bicarbonate/HEPES buffering system which is appropriate for those procedures that do not use a carbon dioxide atmosphere
• Contains the antibiotic Gentamicin
• Contains 5 mg/ml HSA
• For use after pre-equilibration at 37ºC
• Provided in 1ml glass vials
• One-Cell Mouse Embryo tested (>80% 1-cell to expanded blastocyst within 96 hrs)

Store at 2-8ºC
12 month shelf life

For procedures involving the cryopreservation and storage of human sperm used for IUI, IVF and other assisted reproductive procedures

• A HEPES buffered freezing medium for use with human sperm
• Contains 27% glycerol
• For use after pre-equilibration at 37ºC
• Contains 0.4 % HSA
• Provided in 125ml plastic bottle
• One-Cell Mouse Embryo tested (>80% 1-cell to expanded blastocyst within 96 hrs)

Store at 2-8ºC
18 month shelf life

For in vitro sperm washing and isolation procedures which do not require CO2 incubation

• Uses a combined Sodium Bicarbonate/HEPES buffering system which is appropriate for those procedures that do not use a carbon dioxide atmosphere
• Formulated with EDTA and a stabilized form of glutamine
• Contains the antibiotic Gentamicin
• For use without CO2 (pre-equilibrate at 37ºC)
• Contains 5 mg/ml HSA
• Provided in 12x12ml glass vials/box, and both 100ml and 500ml plastic bottles
• One-Cell Mouse Embryo tested (>80% 1-cell to expanded blastocyst within 96 hrs)

Store at 2-8ºC
18 month shelf life